Search for and selects oligonucleotides from a sequence file for polymerase chain reaction.
OLIGO is a multi-functional program that searches for and selects oligonucleotides from a sequence file for polymerase chain reaction (PCR), DNA sequencing, site-directed mutagenesis, and various hybridization applications. It calculates hybridization temperature and secondary structure of oligonucleotides based on the nearest neighbor thermodynamic values. It is also a good tool for construction of synthetic genes, finding an appropriate sequencing primer among those already synthesized, finding and multiplexing consensus prmers and probes, and even finding potential restriction sites in a protein. The OLIGO software has been broadly accepted as the industry software standard for selecting and analyzing oligonucleotides for various molecular biology applications. The first commercial version of the software has been introduced in 1989.
If you are a beginner or an expert in primer design...
andrewc2008
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...you will find it very rewarding<br />This review was originally posted on VersionTracker.com.<br />Oligo 7 is very easy to use and also has great flexibility in various search parameters, so if you are a beginner or an expert in primer design you will find it very rewarding. It has several features not related to primers design, so there is little left to classify it as a general DNA/RNA lab software, and those competitive software packages are prices often twice as high as this one.
Sticking with 4.0 also.
Happy_Pig
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This review was originally posted on VersionTracker.com.<br />I've been using 4.0 for years and to me 700 megs of Classic OS more than offsets $1,200. Even the academic upgrade (i.e. just to run in OSX) from 4->6 for $424 (w/S&H) borders on the ludicrous.
datkinso
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This review was originally posted on VersionTracker.com.<br />The program is quite good (I have used it in a rich laboratory) but we are keeping our old 6100 around to run version 4.