ImageJ for Windows

ImageJ is a free public domain software for processing, analyzing, and visualizing scientific images. It provides tools for image measurement, filtering, segmentation, and transformations, along with drawing and annotation utilities. The platform supports scripting and automation through macros and plugins, enabling repetitive task execution. You can use it to analyze simple microscopy images or complex 3D datasets.

ImageJ has a large ecosystem of third-party plugins that extend functionality for specialized analyses. Variants include ImageJ2 and Fiji, which incorporate backward compatibility and additional libraries while maintaining access to ImageJ 1.x core functions, supporting image data structures and scientific workflows.

Analyze, visualize, process

ImageJ handles image import, export, and display for multiple formats, including grayscale, RGB, and multi-dimensional datasets. It allows measurement of intensity, area, length, and other quantitative properties. You can apply transformations such as rotation, scaling, and cropping, or use filters for noise reduction and edge detection. Image stacks can be processed sequentially or in batch mode, and regions of interest can be defined for localized analysis.

The software provides drawing and annotation tools for marking and labeling images. Macros automate repetitive operations, while scripting supports custom workflows. Plugins extend capabilities, including advanced segmentation, registration, and three-dimensional reconstruction. You can even develop your own plugins to add new features or tailor the software to a specific research task. Standard and custom tools integrate to maintain compatibility with ImageJ 1.x data structures.

Its plugin system enables community-developed extensions to handle specialized analyses, such as particle tracking or fluorescence quantification. However, it relies on community plugins for specialized tasks, as core ImageJ 1.x focuses on general image processing without some advanced built-in algorithms. These structures allow data conversion between versions and integration of third-party algorithms, supporting consistent processing across multiple workflows while preserving the program's original  functionality.

Scientific image tools

ImageJ offers a comprehensive suite of tools for image processing, measurement, visualization, and annotation, enabling scientific analysis. It supports macros, scripting, and plugin-based extensions to execute repetitive or complex operations. ImageJ2 and Fiji are backward-compatible variants that expand upon ImageJ's standard tools for transformations, filters, and quantitative analysis. However, it relies on community plugins for specialized tasks, as core ImageJ 1.x focuses on general image processing without some advanced built-in algorithms.

• Fixed 1.54m9 Analyze>Plot Profile regression where ImageProcessor.getLine() did not sample the correct points